3NF0
mPlum-TTN
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRL BEAMLINE BL12-2 |
| Synchrotron site | SSRL |
| Beamline | BL12-2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2009-02-04 |
| Detector | MAR CCD 165 mm |
| Wavelength(s) | 1.0000 |
| Spacegroup name | P 2 21 21 |
| Unit cell lengths | 38.270, 61.300, 94.240 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 32.463 - 1.750 |
| R-factor | 0.2102 |
| Rwork | 0.208 |
| R-free | 0.25610 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2QLG CHAIN A CHROMOPHORE REMOVED MUTATIONS TRUNCATED. |
| RMSD bond length | 0.047 |
| RMSD bond angle | 2.614 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | PHENIX ((phenix.refine)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 37.400 | 1.840 |
| High resolution limit [Å] | 1.750 | 1.750 |
| Rmerge | 0.089 | 0.360 |
| Number of reflections | 22906 | |
| <I/σ(I)> | 7.6 | 2.6 |
| Completeness [%] | 99.4 | 100 |
| Redundancy | 3.2 | 3.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7 | 298 | 2.1 M malic acid, pH 7.0, VAPOR DIFFUSION, SITTING DROP, temperature 298K |
