3NEZ
mRojoA
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRL BEAMLINE BL12-2 |
| Synchrotron site | SSRL |
| Beamline | BL12-2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2009-02-05 |
| Detector | MAR CCD 165 mm |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 61.240, 97.410, 84.160 |
| Unit cell angles | 90.00, 95.54, 90.00 |
Refinement procedure
| Resolution | 30.480 - 1.700 |
| R-factor | 0.184 |
| Rwork | 0.182 |
| R-free | 0.22100 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2h5q |
| RMSD bond length | 0.023 |
| RMSD bond angle | 2.017 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.5.0088) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 38.000 | 1.790 |
| High resolution limit [Å] | 1.700 | 1.700 |
| Rmerge | 0.042 | 0.393 |
| Number of reflections | 102737 | |
| <I/σ(I)> | 11.9 | 2.2 |
| Completeness [%] | 95.4 | |
| Redundancy | 2.3 | 2.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 6.5 | 298 | 200 mM ammonium acetate, 100 mM Bis-Tris, pH 6.5, and 25% (w/v) polyethylene glycol 3350, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
