3NET
Crystal structure of histidyl-tRNA synthetase from Nostoc sp. PCC 7120
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-ID |
| Synchrotron site | APS |
| Beamline | 19-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2010-02-08 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.97937 |
| Spacegroup name | P 43 2 2 |
| Unit cell lengths | 73.656, 73.656, 521.912 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 50.000 - 2.700 |
| R-factor | 0.21926 |
| Rwork | 0.217 |
| R-free | 0.26545 |
| Structure solution method | SAD |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.206 |
| Data reduction software | HKL-3000 |
| Data scaling software | HKL-3000 |
| Phasing software | HKL-3000 |
| Refinement software | REFMAC (5.5.0109) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.720 |
| High resolution limit [Å] | 2.700 | 2.700 |
| Rmerge | 0.095 | 0.618 |
| Number of reflections | 38504 | |
| <I/σ(I)> | 29.6 | 3.67 |
| Completeness [%] | 93.1 | 100 |
| Redundancy | 8.3 | 9.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6.5 | 297 | 0.2M calicium Acetate, 0.1M Sodium cacodylate, 40% PEG 300, pH 6.5, VAPOR DIFFUSION, SITTING DROP, temperature 297K |
