3NEJ
Q28E mutant of Hera RNA helicase N-terminal domain - perfectly twinned hexagonal form
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X10SA |
| Synchrotron site | SLS |
| Beamline | X10SA |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2010-05-31 |
| Detector | PSI PILATUS 6M |
| Wavelength(s) | 1 |
| Spacegroup name | P 31 2 1 |
| Unit cell lengths | 59.689, 59.689, 239.852 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 39.975 - 2.570 |
| R-factor | 0.2143 |
| Rwork | 0.212 |
| R-free | 0.26850 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2gxs |
| RMSD bond length | 0.004 |
| RMSD bond angle | 0.868 |
| Data reduction software | XDS |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | PHENIX ((phenix.refine: 1.6_289)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 43.400 | 2.700 |
| High resolution limit [Å] | 2.570 | 2.600 |
| Number of reflections | 15772 | |
| <I/σ(I)> | 7.2 | 1.2 |
| Completeness [%] | 94.2 | 86.4 |
| Redundancy | 4 | 2.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 298 | 15% PEG3350, 0.2M sodium malate, 0.1M HEPES/NaOH pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
