3NE5
Crystal structure of the CusBA heavy-metal efflux complex from Escherichia coli
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 24-ID-C |
| Synchrotron site | APS |
| Beamline | 24-ID-C |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2010-04-09 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.9792 |
| Spacegroup name | H 3 2 |
| Unit cell lengths | 160.267, 160.267, 682.835 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 39.493 - 2.898 |
| R-factor | 0.231 |
| Rwork | 0.229 |
| R-free | 0.26700 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.003 |
| RMSD bond angle | 0.683 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | PHASER |
| Refinement software | PHENIX |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 39.496 | 3.000 |
| High resolution limit [Å] | 2.898 | 2.898 |
| Rmerge | 0.090 | 0.493 |
| Number of reflections | 75498 | |
| <I/σ(I)> | 1.5 | |
| Completeness [%] | 93.2 | 90.9 |
| Redundancy | 1.4 | 1.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | EVAPORATION | 7.5 | 298 | 10 % PEG3350, 0.4M NH4SO4, 1% JM600, 5% Glycerol, 0.1M Bis-tris propane (7.5), EVAPORATION, temperature 298K |
