3NE2
Archaeoglobus fulgidus aquaporin
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 8.3.1 |
| Synchrotron site | ALS |
| Beamline | 8.3.1 |
| Temperature [K] | 103 |
| Detector technology | CCD |
| Collection date | 2007-04-04 |
| Detector | ADSC QUANTUM 210 |
| Wavelength(s) | 1.1159 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 84.899, 199.861, 90.567 |
| Unit cell angles | 90.00, 117.51, 90.00 |
Refinement procedure
| Resolution | 39.070 - 3.000 |
| R-factor | 0.246 |
| Rwork | 0.245 |
| R-free | 0.27400 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | PDB Accession code 2F2B |
| RMSD bond length | 0.012 |
| RMSD bond angle | 1.165 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | MOLREP |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 40.000 | 2.800 |
| High resolution limit [Å] | 2.800 | 2.800 |
| Rmerge | 0.077 | 0.200 |
| Number of reflections | 35301 | |
| <I/σ(I)> | 7.6 | 2.4 |
| Completeness [%] | 54.6 | 12.7 |
| Redundancy | 2.3 | 2.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 |
