3NDQ
Structure of Human TFIIS Domain II
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL17U |
| Synchrotron site | SSRF |
| Beamline | BL17U |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2010-04-01 |
| Detector | RAYONIX MX-225 |
| Wavelength(s) | 0.97947 |
| Spacegroup name | I 2 2 2 |
| Unit cell lengths | 59.270, 60.750, 61.980 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 21.693 - 1.933 |
| R-factor | 0.168 |
| Rwork | 0.166 |
| R-free | 0.20800 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2dme |
| RMSD bond length | 0.006 |
| RMSD bond angle | 0.894 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | MLPHARE |
| Refinement software | PHENIX (1.6.1_357) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 43.385 | 21.690 | 2.040 |
| High resolution limit [Å] | 1.933 | 6.110 | 1.933 |
| Rmerge | 0.079 | 0.061 | 0.110 |
| Total number of observations | 2508 | 17758 | |
| Number of reflections | 8564 | ||
| <I/σ(I)> | 27 | 10.6 | 5 |
| Completeness [%] | 99.0 | 74.2 | 100 |
| Redundancy | 14.2 | 11 | 14.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 289 | 1.0 M Ammonium sulphate, 0.1 M 2-(N-morpholino)ethanesulfonic acid , pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 289K |
