3NDK
RB69 DNA Polymerase (Y567A) Ternary Complex with dCTP Opposite dG
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X29A |
| Synchrotron site | NSLS |
| Beamline | X29A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2009-11-09 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 1.07500 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 75.006, 120.015, 130.781 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 50.000 - 2.000 |
| R-factor | 0.189 |
| Rwork | 0.187 |
| R-free | 0.22865 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3nci |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.023 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | AMoRE |
| Refinement software | REFMAC (5.5.0102) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.070 |
| High resolution limit [Å] | 2.000 | 2.000 |
| Rmerge | 0.140 | 0.879 |
| Number of reflections | 76738 | |
| <I/σ(I)> | 8.5 | 1.18 |
| Completeness [%] | 95.3 | 96 |
| Redundancy | 3.3 | 3.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | micro-batch vapor-diffusion | 6.5 | 293 | 10% (w/v) PEG 350 monomethyl ether (MME), 175 mM CaCl2, 100 mM NaCacodylate (pH 6.5), micro-batch vapor-diffusion, temperature 293K |
