3NDC
Crystal structure of Precorrin-4 C11-methyltransferase from Rhodobacter capsulatus
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SRS BEAMLINE PX10.1 |
| Synchrotron site | SRS |
| Beamline | PX10.1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2007-11-15 |
| Detector | MARMOSAIC 225 mm CCD |
| Wavelength(s) | 1.117 |
| Spacegroup name | P 65 |
| Unit cell lengths | 84.517, 84.517, 144.637 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 40.560 - 2.000 |
| R-factor | 0.18875 |
| Rwork | 0.187 |
| R-free | 0.22190 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1cbf |
| RMSD bond length | 0.012 |
| RMSD bond angle | 1.344 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.5.0102) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 42.260 | 2.110 |
| High resolution limit [Å] | 2.000 | 2.000 |
| Rmerge | 0.068 | 0.620 |
| Number of reflections | 39472 | |
| <I/σ(I)> | 19.2 | 3.3 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 9.8 | 8.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 292 | 0.1M Hepes pH 7.5, 10% isopropanol, 20% PEG 4000, VAPOR DIFFUSION, HANGING DROP, temperature 292K |
