3NDB
Crystal structure of a signal sequence bound to the signal recognition particle
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID23-1 |
| Synchrotron site | ESRF |
| Beamline | ID23-1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2009-02-09 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 1.0 |
| Spacegroup name | I 2 2 2 |
| Unit cell lengths | 108.835, 126.275, 201.758 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 20.000 - 3.000 |
| R-factor | 0.23003 |
| Rwork | 0.228 |
| R-free | 0.26652 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2v3c |
| RMSD bond length | 0.014 |
| RMSD bond angle | 2.068 |
| Data scaling software | SCALA |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.5.0109) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 63.800 |
| High resolution limit [Å] | 3.000 |
| Rmerge | 0.102 |
| Number of reflections | 27778 |
| Completeness [%] | 98.5 |
| Redundancy | 4.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | EVAPORATION | 5.6 | 291 | 30% MPD, 200 mM NH4PO4 (pH 5.6), EVAPORATION, temperature 291K |
