3ND4
Watson-Crick 16-mer dsRNA
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU RUH3R |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2008-11-28 |
| Detector | RIGAKU RAXIS IV |
| Wavelength(s) | 1.5418 |
| Spacegroup name | H 3 2 |
| Unit cell lengths | 41.094, 41.094, 123.690 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 18.390 - 1.524 |
| R-factor | 0.176 |
| Rwork | 0.172 |
| R-free | 0.21400 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.018 |
| RMSD bond angle | 1.614 |
| Data scaling software | SCALA (3.3.15) |
| Phasing software | MOLREP |
| Refinement software | PHENIX (1.6.1_357) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 18.390 | 18.390 | 1.610 |
| High resolution limit [Å] | 1.524 | 4.820 | 1.520 |
| Rmerge | 0.033 | 0.158 | |
| Total number of observations | 1659 | 4063 | |
| Number of reflections | 6419 | ||
| <I/σ(I)> | 26 | 14.3 | 4.7 |
| Completeness [%] | 99.5 | 98.5 | 97.1 |
| Redundancy | 7.3 | 7.1 | 4.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 298 | 15 mM MgCl2, 10 mM Spermine, 2M lithium sulfate, pH 6.5, vapor diffusion, hanging drop, temperature 298K |
