3ND0
X-ray crystal structure of a slow cyanobacterial Cl-/H+ antiporter
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X29A |
| Synchrotron site | NSLS |
| Beamline | X29A |
| Temperature [K] | 78 |
| Detector technology | CCD |
| Collection date | 2008-03-23 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 1.0809 |
| Spacegroup name | P 3 2 1 |
| Unit cell lengths | 203.790, 203.790, 96.820 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 65.219 - 3.200 |
| R-factor | 0.2439 |
| Rwork | 0.243 |
| R-free | 0.26880 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.003 |
| RMSD bond angle | 0.765 |
| Data scaling software | SCALA (3.3.16) |
| Phasing software | PHASER (2.1.4) |
| Refinement software | PHENIX (1.6.1_357) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 67.000 |
| High resolution limit [Å] | 3.200 |
| Number of reflections | 35234 |
| Completeness [%] | 92.0 |
| Redundancy | 15.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8 | 293 | 30 % PEG 400, 200 mM CaCl2 , Tris-HCl 100 mM pH 8, vapor diffusion, hanging drop, temperature 293K |
