3NCR
GlnK2 from Archaeoglubus fulgidus, ADP complex
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | EMBL/DESY, HAMBURG BEAMLINE X12 |
| Synchrotron site | EMBL/DESY, HAMBURG |
| Beamline | X12 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Detector | MARMOSAIC 225 mm CCD |
| Wavelength(s) | 1.00 |
| Spacegroup name | I 4 2 2 |
| Unit cell lengths | 123.830, 123.830, 125.459 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 39.620 - 1.440 |
| R-factor | 0.15899 |
| Rwork | 0.157 |
| R-free | 0.19950 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3ncp |
| RMSD bond length | 0.034 |
| RMSD bond angle | 2.380 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.5.0109) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 43.700 | 1.540 |
| High resolution limit [Å] | 1.440 | 1.440 |
| Rmerge | 0.094 | 0.699 |
| Number of reflections | 88498 | |
| <I/σ(I)> | 8.82 | 2.13 |
| Completeness [%] | 99.8 | 98.8 |
| Redundancy | 6.97 | 6.08 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 5.6 | 293 | ammonium phosphate, Tris-HCl, pH 5.6, VAPOR DIFFUSION, SITTING DROP, temperature 293K |
