3NCP
GlnK2 from Archaeoglobus fulgidus
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | EMBL/DESY, HAMBURG BEAMLINE X12 |
| Synchrotron site | EMBL/DESY, HAMBURG |
| Beamline | X12 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Detector | MARMOSAIC 225 mm CCD |
| Wavelength(s) | 1.00 |
| Spacegroup name | P 3 |
| Unit cell lengths | 91.000, 91.000, 64.432 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 37.170 - 2.350 |
| R-factor | 0.2018 |
| Rwork | 0.201 |
| R-free | 0.22000 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2gnk |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.360 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | MOLREP |
| Refinement software | BUSTER (2.9.4) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 45.500 | 2.440 |
| High resolution limit [Å] | 2.350 | 2.350 |
| Rmerge | 0.061 | 0.366 |
| Number of reflections | 24887 | |
| <I/σ(I)> | 6.38 | 2.91 |
| Completeness [%] | 99.8 | 99.5 |
| Redundancy | 2.45 | 2.49 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 8 | 293 | PEG 4000, Na Acetate, pH 8.0, VAPOR DIFFUSION, SITTING DROP, temperature 293K |
