3NCJ
Crystal structure of Fab15 Mut8
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU MICROMAX-007 HF |
| Temperature [K] | 93 |
| Detector technology | CCD |
| Collection date | 2010-06-03 |
| Detector | RIGAKU SATURN 944 |
| Wavelength(s) | 1.5418 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 54.550, 74.700, 65.390 |
| Unit cell angles | 90.00, 104.23, 90.00 |
Refinement procedure
| Resolution | 29.175 - 1.600 |
| R-factor | 0.2017 |
| Rwork | 0.200 |
| R-free | 0.22450 |
| Structure solution method | FOURIER SYNTHESIS |
| Starting model (for MR) | 3naa |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.095 |
| Data reduction software | d*TREK |
| Data scaling software | d*TREK |
| Refinement software | PHENIX ((phenix.refine: 1.6.1_357)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 1.660 |
| High resolution limit [Å] | 1.600 | 1.600 |
| Rmerge | 0.061 | 0.230 |
| Number of reflections | 60088 | |
| <I/σ(I)> | 16 | 2.2 |
| Completeness [%] | 89.2 | 53.9 |
| Redundancy | 4 | 1.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 4.5 | 293 | 0.1 sodium acetate, 7% PEG 5000, 0.2 M zinc acetate, 5% dioxane, pH 4.5, VAPOR DIFFUSION, SITTING DROP, temperature 293K |
