3NCH
Yeast Glycogen Synthase (Gsy2p) Basal State Conformation
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-ID |
| Synchrotron site | APS |
| Beamline | 19-ID |
| Temperature [K] | 100 |
| Wavelength(s) | 1.01 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 93.940, 161.837, 121.622 |
| Unit cell angles | 90.00, 95.82, 90.00 |
Refinement procedure
| Resolution | 46.728 - 2.880 |
| R-factor | 0.2154 |
| Rwork | 0.213 |
| R-free | 0.26480 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3naz |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.142 |
| Data reduction software | HKL-3000 |
| Data scaling software | HKL-3000 |
| Phasing software | AMoRE |
| Refinement software | PHENIX ((phenix.refine)) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 50.000 |
| High resolution limit [Å] | 2.880 |
| Rmerge | 0.087 |
| Number of reflections | 80388 |
| <I/σ(I)> | 17.1 |
| Completeness [%] | 99.0 |
| Redundancy | 4.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8.5 | 293 | 0.1 M Tris pH 8.5, 0.2 M Lithium sulfate, 20% PEG 3400, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
