3NCB
A mutant human Prolactin receptor antagonist H180A in complex with the extracellular domain of the human prolactin receptor
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X29A |
| Synchrotron site | NSLS |
| Beamline | X29A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2010-05-02 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 1.075 |
| Spacegroup name | P 65 |
| Unit cell lengths | 124.210, 124.210, 71.556 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 32.060 - 2.100 |
| R-factor | 0.1881 |
| Rwork | 0.186 |
| R-free | 0.23743 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3mzg |
| RMSD bond length | 0.026 |
| RMSD bond angle | 2.139 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | PHASER (2.1.4) |
| Refinement software | REFMAC (5.5.0109) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 33.000 | 33.000 | 2.020 |
| High resolution limit [Å] | 1.950 | 4.200 | 1.950 |
| Rmerge | 0.090 | 0.056 | 0.644 |
| Number of reflections | 46104 | ||
| <I/σ(I)> | 11.4 | ||
| Completeness [%] | 99.7 | 99.2 | 98.2 |
| Redundancy | 10.8 | 10.3 | 8.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 291 | 3.7M NaCl, 0.1 M Hepes 7.5, 6% PEG monomethylether 550, VAPOR DIFFUSION, HANGING DROP, temperature 291K |
