3NBU
Crystal structure of pGI glucosephosphate isomerase
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 8.3.1 |
| Synchrotron site | ALS |
| Beamline | 8.3.1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2007-10-20 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 1.11587 |
| Spacegroup name | P 1 |
| Unit cell lengths | 69.806, 72.874, 181.851 |
| Unit cell angles | 92.47, 97.82, 114.57 |
Refinement procedure
| Resolution | 89.000 - 2.050 |
| R-factor | 0.17227 |
| Rwork | 0.169 |
| R-free | 0.22952 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1dqr |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.313 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.5.0102) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 89.000 | 2.100 |
| High resolution limit [Å] | 1.990 | 1.990 |
| Rmerge | 0.104 | 0.423 |
| Number of reflections | 208294 | |
| <I/σ(I)> | 9.7 | 1.7 |
| Completeness [%] | 94.6 | 94.6 |
| Redundancy | 2 | 2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 5.5 | 298 | 25% PEG 3350, 0.2M ammonium acetate, 0.1M Bis-Tris 5.5, 10 mM betaine HCl, VAPOR DIFFUSION, SITTING DROP, temperature 298K |
