3NBF
Q28E mutant of hera helicase N-terminal domain bound to 8-oxo-ADP
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X10SA |
| Synchrotron site | SLS |
| Beamline | X10SA |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2010-05-31 |
| Detector | PSI PILATUS 6M |
| Wavelength(s) | 1 |
| Spacegroup name | P 1 |
| Unit cell lengths | 60.648, 60.676, 74.085 |
| Unit cell angles | 68.90, 77.29, 72.64 |
Refinement procedure
| Resolution | 41.512 - 1.900 |
| R-factor | 0.1861 |
| Rwork | 0.184 |
| R-free | 0.22180 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2gxs |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.092 |
| Data reduction software | XDS |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | PHENIX ((phenix.refine: 1.6_289)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 46.500 | 1.930 |
| High resolution limit [Å] | 1.900 | 1.900 |
| Number of reflections | 70329 | |
| <I/σ(I)> | 7.6 | 1.2 |
| Completeness [%] | 95.7 | 94.6 |
| Redundancy | 2.4 | 2.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 310 | 15% PEG 3350, 0.2M sodium citrate, 0.1M HEPES/NaOH pH 7.5, VAPOR DIFFUSION, SITTING DROP, temperature 310K |
