3NBC
Clitocybe nebularis ricin B-like lectin (CNL) in complex with lactose, crystallized at pH 4.4
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ELETTRA BEAMLINE 5.2R |
| Synchrotron site | ELETTRA |
| Beamline | 5.2R |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2009-02-18 |
| Detector | MAR CCD 165 mm |
| Wavelength(s) | 1.033 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 54.789, 55.898, 112.414 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 27.950 - 1.010 |
| R-factor | 0.158 |
| Rwork | 0.157 |
| R-free | 0.17200 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | The starting model was partial structure obtained by iodine SAD phasing of unpublished data set. |
| RMSD bond length | 0.051 |
| RMSD bond angle | 2.105 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | AMoRE |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.030 |
| High resolution limit [Å] | 1.010 | 1.010 |
| Rmerge | 0.046 | 0.403 |
| Number of reflections | 177749 | |
| <I/σ(I)> | 17.5 | |
| Completeness [%] | 98.4 | 94.8 |
| Redundancy | 8.7 | 5.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 4.4 | 293 | 0.1M Sodium acetate, 29%(v/v) PEG 550 MME, pH 4.4, VAPOR DIFFUSION, SITTING DROP, temperature 293K |
