3NAZ
Basal state form of Yeast Glycogen Synthase
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 23-ID-B |
| Synchrotron site | APS |
| Beamline | 23-ID-B |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2008-10-30 |
| Detector | MARMOSAIC 300 mm CCD |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 96.529, 166.154, 121.029 |
| Unit cell angles | 90.00, 103.39, 90.00 |
Refinement procedure
| Resolution | 48.030 - 3.000 |
| R-factor | 0.205 |
| Rwork | 0.203 |
| R-free | 0.25400 |
| Structure solution method | MIR |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.068 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | SOLVE |
| Refinement software | PHENIX |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 50.000 |
| High resolution limit [Å] | 3.000 |
| Rmerge | 0.082 |
| Number of reflections | 73853 |
| <I/σ(I)> | 13.5 |
| Completeness [%] | 99.6 |
| Redundancy | 3.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 8.5 | 293 | 0.1 M Tris-HCl pH 8.5, 20% PEG 3400, 0.2 M lithium sulfate, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
