3NAS
The crystal structure of beta-phosphoglucomutase from Bacillus subtilis
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X29A |
| Synchrotron site | NSLS |
| Beamline | X29A |
| Temperature [K] | 110 |
| Detector technology | CCD |
| Collection date | 2010-01-12 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.9792 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 67.043, 78.171, 92.067 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 59.589 - 3.000 |
| R-factor | 0.2355 |
| Rwork | 0.233 |
| R-free | 0.29020 |
| Structure solution method | SAD |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.052 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | SOLVE |
| Refinement software | PHENIX ((phenix.refine)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 92.070 | 2.580 |
| High resolution limit [Å] | 2.450 | 2.450 |
| Rmerge | 0.075 | 0.640 |
| Number of reflections | 18400 | |
| <I/σ(I)> | 22.1 | 4.2 |
| Completeness [%] | 99.9 | 100 |
| Redundancy | 13.7 | 14.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6.5 | 291 | 0.1 M BIS-TRIS pH 6.5, 3.0 M Sodium chloride., VAPOR DIFFUSION, SITTING DROP, temperature 291K |
