3NAR
Crystal structure of ZHX1 HD4 (zinc-fingers and homeoboxes protein 1, homeodomain 4)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2005-01-14 |
| Detector | MAR scanner 345 mm plate |
| Wavelength(s) | 1.541 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 64.900, 48.800, 49.320 |
| Unit cell angles | 90.00, 104.02, 90.00 |
Refinement procedure
| Resolution | 19.450 - 2.600 |
| R-factor | 0.20149 |
| Rwork | 0.199 |
| R-free | 0.25039 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2ecc |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.021 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.6.0077) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 2.690 |
| High resolution limit [Å] | 2.600 | 2.600 |
| Rmerge | 0.102 | 0.339 |
| Number of reflections | 4674 | |
| <I/σ(I)> | 24.3 | 5.5 |
| Completeness [%] | 99.7 | |
| Redundancy | 8.3 | 6.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5.6 | 294 | 2.5M ammonium sulphate, 10mM magnesium acetate, MES pH5.6, 20mM ATP, 20mM phenol, VAPOR DIFFUSION, HANGING DROP, temperature 294K |
