3NAH
Crystal structures and functional analysis of murine norovirus RNA-dependent RNA polymerase
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PAL/PLS BEAMLINE 6C1 |
| Synchrotron site | PAL/PLS |
| Beamline | 6C1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2009-10-15 |
| Detector | ADSC QUANTUM 210 |
| Wavelength(s) | 1.239 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 120.833, 196.543, 109.156 |
| Unit cell angles | 90.00, 114.21, 90.00 |
Refinement procedure
| Resolution | 44.060 - 2.750 |
| R-factor | 0.225 |
| Rwork | 0.221 |
| R-free | 0.30300 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.012 |
| RMSD bond angle | 1.506 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.5.0109) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.900 |
| High resolution limit [Å] | 2.750 | 2.800 |
| Rmerge | 0.186 | 0.433 |
| Number of reflections | 55441 | |
| <I/σ(I)> | 4.3 | |
| Completeness [%] | 94.0 | 76.3 |
| Redundancy | 4.3 | 2.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 1M (NH4)2SO4, 100mM cacodylate (pH 6.5), VAPOR DIFFUSION, HANGING DROP |
