3NAC
Crystal structure of Fab15 Mut7
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 17-ID |
| Synchrotron site | APS |
| Beamline | 17-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2010-03-17 |
| Detector | ADSC QUANTUM 210 |
| Wavelength(s) | 1.0000 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 54.663, 74.437, 65.764 |
| Unit cell angles | 90.00, 104.25, 90.00 |
Refinement procedure
| Resolution | 36.560 - 1.800 |
| R-factor | 0.1859 |
| Rwork | 0.184 |
| R-free | 0.21170 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3na9 |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.094 |
| Data reduction software | d*TREK |
| Data scaling software | d*TREK |
| Phasing software | PHASER |
| Refinement software | PHENIX ((phenix.refine: 1.6.1_357)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 46.800 | 1.860 |
| High resolution limit [Å] | 1.800 | 1.800 |
| Rmerge | 0.082 | 0.396 |
| Number of reflections | 46820 | |
| <I/σ(I)> | 7.6 | 2.2 |
| Completeness [%] | 99.3 | 99.8 |
| Redundancy | 3.6 | 3.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6 | 293 | 0.1 M sodium acetate, 12% PEG MME 5000, 0.2 M zinc acetate, pH 6.0, VAPOR DIFFUSION, SITTING DROP, temperature 293K |
