3NAB
Crystal Structure of fab15 Mut6
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 17-ID |
| Synchrotron site | APS |
| Beamline | 17-ID |
| Detector technology | CCD |
| Collection date | 2010-03-17 |
| Detector | ADSC QUANTUM 210 |
| Wavelength(s) | 1.0000 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 54.618, 75.197, 67.226 |
| Unit cell angles | 90.00, 108.93, 90.00 |
Refinement procedure
| Resolution | 34.935 - 2.320 |
| R-factor | 0.2064 |
| Rwork | 0.203 |
| R-free | 0.25920 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3na9 |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.082 |
| Data reduction software | d*TREK |
| Data scaling software | d*TREK |
| Phasing software | PHASER |
| Refinement software | PHENIX ((phenix.refine: 1.6.1_357)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 42.600 | 2.400 |
| High resolution limit [Å] | 2.320 | 2.320 |
| Rmerge | 0.083 | 0.323 |
| Number of reflections | 22257 | |
| <I/σ(I)> | 8.6 | 2.5 |
| Completeness [%] | 99.3 | 100 |
| Redundancy | 3.64 | 3.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 4.5 | 293 | 0.1 M sodium acetate, 12% PEG MME 5000, 0.2 M Zn acetate, pH 4.5, VAPOR DIFFUSION, SITTING DROP, temperature 293K |
