3NA8
Crystal Structure of a putative dihydrodipicolinate synthetase from Pseudomonas aeruginosa
Experimental procedure
| Experimental method | MAD |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 17-ID |
| Synchrotron site | APS |
| Beamline | 17-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2006-10-08 |
| Detector | ADSC QUANTUM 210r |
| Wavelength(s) | 0.97937 |
| Spacegroup name | P 21 21 2 |
| Unit cell lengths | 107.370, 156.210, 76.580 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 43.060 - 1.850 |
| R-factor | 0.23674 |
| Rwork | 0.235 |
| R-free | 0.26972 |
| Structure solution method | SAD |
| RMSD bond length | 0.002 |
| RMSD bond angle | 0.527 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | SHARP |
| Refinement software | REFMAC (5.5.0066) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 48.730 | 1.940 |
| High resolution limit [Å] | 1.850 | 1.850 |
| Rmerge | 0.104 | 0.714 |
| Number of reflections | 106856 | |
| <I/σ(I)> | 11.89 | 1.99 |
| Completeness [%] | 96.5 | 90.9 |
| Redundancy | 7.27 | 6.78 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 7 | 293 | 17% PEG6000, 0.4M Malic Acid, pH 7.0, VAPOR DIFFUSION, temperature 293K |
