3NA7
2.2 Angstrom Structure of the HP0958 Protein from Helicobacter pylori CCUG 17874
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | CLSI BEAMLINE 08ID-1 |
| Synchrotron site | CLSI |
| Beamline | 08ID-1 |
| Temperature [K] | 77 |
| Detector technology | CCD |
| Collection date | 2008-07-24 |
| Detector | MARMOSAIC 225 mm CCD |
| Wavelength(s) | 0.984 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 172.720, 37.951, 66.056 |
| Unit cell angles | 90.00, 110.66, 90.00 |
Refinement procedure
| Resolution | 30.000 - 2.200 |
| R-factor | 0.25067 |
| Rwork | 0.248 |
| R-free | 0.29378 |
| Structure solution method | SAD |
| RMSD bond length | 0.012 |
| RMSD bond angle | 1.316 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | SOLVE |
| Refinement software | REFMAC (5.5.0109) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.280 |
| High resolution limit [Å] | 2.200 | 2.200 |
| Rmerge | 0.056 | 0.220 |
| Number of reflections | 19056 | |
| <I/σ(I)> | 33.5 | 3.5 |
| Completeness [%] | 91.6 | 65.7 |
| Redundancy | 5 | 3.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 298 | 18% PEG 3350, 0.1M HEPES pH 7.5, 0.2M magnesium acetate, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
