3N9X
Crystal structure of Map Kinase from plasmodium berghei, PB000659.00.0
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU FR-E+ SUPERBRIGHT |
| Detector technology | CCD |
| Collection date | 2010-05-19 |
| Detector | RIGAKU SATURN A200 |
| Wavelength(s) | 1.5418 |
| Spacegroup name | P 21 21 2 |
| Unit cell lengths | 112.315, 120.727, 96.499 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 32.170 - 2.050 |
| R-factor | 0.193 |
| Rwork | 0.191 |
| R-free | 0.22900 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.079 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | PHASER (2.1.4) |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.090 |
| High resolution limit [Å] | 2.050 | 2.050 |
| Rmerge | 0.051 | 0.617 |
| Number of reflections | 82807 | |
| <I/σ(I)> | 6.8 | 1.38 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 7.2 | 7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 293 | 25% glycerol, 20 % peg3350, 0.2 M di sodium tartrate, 2 mM ATP, 4 mM MgCl2, 2 mM TCEP, pH 7.5, VAPOR DIFFUSION, SITTING DROP, temperature 293K |
