3N9V
Crystal Structure of INPP5B
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I03 |
| Synchrotron site | Diamond |
| Beamline | I03 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2009-10-23 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.96860 |
| Spacegroup name | I 21 21 21 |
| Unit cell lengths | 96.160, 110.660, 159.380 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 53.661 - 2.650 |
| R-factor | 0.1914 |
| Rwork | 0.190 |
| R-free | 0.23080 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1i9y |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.257 |
| Data reduction software | iMOSFLM |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | PHENIX ((phenix.refine: 1.6_289)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 72.548 | 2.793 |
| High resolution limit [Å] | 2.600 | 2.600 |
| Rmerge | 0.077 | 0.620 |
| Number of reflections | 25062 | |
| <I/σ(I)> | 11.9 | 2.1 |
| Completeness [%] | 99.9 | 99.9 |
| Redundancy | 4.6 | 4.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 8.5 | 293 | 0.2M Magnesium Chloride, 0.1M Tris-HCl pH 8.5, 20% PEG 8000, VAPOR DIFFUSION, SITTING DROP, temperature 293K |
