3N9N

ceKDM7A from C.elegans, complex with H3K4me3K9me2 peptide and NOG

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL17U
Synchrotron site	SSRF
Beamline	BL17U
Temperature [K]	100
Detector technology	CCD
Collection date	2009-05-06
Detector	RAYONIX MX-225
Wavelength(s)	0.9792
Spacegroup name	P 21 21 21
Unit cell lengths	68.400, 85.543, 102.550
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	39.476 - 2.299
R-factor	0.1918
Rwork	0.189
R-free	0.24030
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3n9m
RMSD bond length	0.005
RMSD bond angle	0.915
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASES
Refinement software	PHENIX ((phenix.refine))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.380
High resolution limit [Å]	2.299	2.299
Total number of observations	395510
Number of reflections	27466
Completeness [%]	100.0	100
Redundancy		14.4

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7	277	16% PEG 3350, 0.2M Sodium Fluoride, 0.1M Bis-Tris or HEPES, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 277K