3N9I
Crystal structure of tryptophanyl-tRNA synthetase from Yersinia pestis CO92
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-ID |
| Synchrotron site | APS |
| Beamline | 19-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2010-04-09 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.9794 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 61.099, 78.150, 152.176 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 40.000 - 1.950 |
| R-factor | 0.16905 |
| Rwork | 0.167 |
| R-free | 0.20615 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3fi0 |
| RMSD bond length | 0.016 |
| RMSD bond angle | 1.420 |
| Data reduction software | HKL-3000 |
| Data scaling software | HKL-3000 |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.5.0109) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 40.000 | 1.980 |
| High resolution limit [Å] | 1.950 | 1.950 |
| Rmerge | 0.077 | 0.650 |
| Number of reflections | 53290 | |
| <I/σ(I)> | 9.3 | 2.8 |
| Completeness [%] | 99.2 | 98.4 |
| Redundancy | 5.4 | 5.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7 | 291 | 0.2 M Ca(oAc)2 0.1 M Tris 20% PEG3000, pH 7.0, VAPOR DIFFUSION, SITTING DROP, temperature 291K |
