3N9E
Mite-y Lysozyme: Promite
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | AUSTRALIAN SYNCHROTRON BEAMLINE MX1 |
| Synchrotron site | Australian Synchrotron |
| Beamline | MX1 |
| Temperature [K] | 293 |
| Detector technology | CCD |
| Collection date | 2009-07-29 |
| Detector | ADSC QUANTUM 210 |
| Wavelength(s) | 0.98 |
| Spacegroup name | P 43 21 2 |
| Unit cell lengths | 79.285, 79.285, 38.003 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 34.270 - 1.380 |
| R-factor | 0.16342 |
| Rwork | 0.162 |
| R-free | 0.18315 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2blx |
| RMSD bond length | 0.029 |
| RMSD bond angle | 2.702 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.6.0062) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 39.700 | 1.450 |
| High resolution limit [Å] | 1.380 | 1.380 |
| Rmerge | 0.054 | 0.474 |
| Number of reflections | 25326 | |
| <I/σ(I)> | 35.1 | 4.8 |
| Completeness [%] | 99.4 | 96 |
| Redundancy | 19.5 | 11.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | LIQUID DIFFUSION | 4.5 | 293 | Crystals were obtained by counter diffusion against Promite, pH 4.5, LIQUID DIFFUSION, temperature 293K |
