3N7E
Crystal structure of CopK bound to Cu(II)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 23-ID-D |
| Synchrotron site | APS |
| Beamline | 23-ID-D |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2008-08-03 |
| Detector | MARMOSAIC 300 mm CCD |
| Wavelength(s) | 1.3776 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 49.835, 51.593, 52.054 |
| Unit cell angles | 90.00, 116.91, 90.00 |
Refinement procedure
| Resolution | 46.419 - 2.295 |
| R-factor | 0.206 |
| Rwork | 0.199 |
| R-free | 0.26700 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3dso |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.211 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.5_2) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.380 |
| High resolution limit [Å] | 2.295 | 2.295 |
| Rmerge | 0.070 | 0.233 |
| Number of reflections | 5184 | |
| <I/σ(I)> | 16.5 | 5.2 |
| Completeness [%] | 96.7 | 91 |
| Redundancy | 5.2 | 3.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 298 | 0.2M KNO3, 24% (w/v) PEG 3350, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
