3N73
Crystal structure of a putative 4-hydroxy-2-oxoglutarate aldolase from Bacillus cereus
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-ID |
| Synchrotron site | APS |
| Beamline | 19-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2008-07-11 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.9793 |
| Spacegroup name | P 21 21 2 |
| Unit cell lengths | 81.066, 166.644, 37.279 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 50.000 - 2.070 |
| R-factor | 0.17161 |
| Rwork | 0.169 |
| R-free | 0.22128 |
| Structure solution method | SAD |
| RMSD bond length | 0.016 |
| RMSD bond angle | 1.441 |
| Data reduction software | HKL-3000 |
| Data scaling software | HKL-2000 |
| Phasing software | HKL-3000 |
| Refinement software | REFMAC (5.5.0109) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.090 |
| High resolution limit [Å] | 2.050 | 2.050 |
| Rmerge | 0.108 | 0.673 |
| Number of reflections | 30820 | |
| <I/σ(I)> | 26.371 | 3.3 |
| Completeness [%] | 99.3 | 66.6 |
| Redundancy | 7.7 | 7.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 6.5 | 295 | 0.1M BIS-TRIS PH6.5, 0.2M NACL, 25% PEG3350, VAPOR DIFFUSION - HANGING DROP, TEMPERATURE 273K, temperature 295K |
