3N72

Crystal Structure of Aha-1 from plasmodium falciparum, PFC0270w

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU FR-E+ SUPERBRIGHT
Temperature [K]	100
Detector technology	IMAGE PLATE
Collection date	2010-04-19
Detector	RIGAKU RAXIS IV++
Wavelength(s)	1.5418
Spacegroup name	P 31
Unit cell lengths	63.709, 63.709, 61.722
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	50.000 - 1.770
R-factor	0.191
Rwork	0.188
R-free	0.24700
Structure solution method	SAD
RMSD bond length	0.012
RMSD bond angle	1.320
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	SHELX
Refinement software	REFMAC

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	1.800
High resolution limit [Å]	1.770	1.770
Rmerge	0.059	0.519
Number of reflections	27245
<I/σ(I)>	12.8	3.4
Completeness [%]	99.8	96.8
Redundancy	5.6	5.2

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7.5	293	10 mM Hepes, 30% PEG 2000 MME, 0.2 M Potassium thiocyanate, pH 7.5, VAPOR DIFFUSION, SITTING DROP, temperature 293K
1	VAPOR DIFFUSION, SITTING DROP	7.5	293	10 mM Hepes, 30% PEG 2000 MME, 0.2 M Potassium thiocyanate, pH 7.5, VAPOR DIFFUSION, SITTING DROP, temperature 293K