3N6Z

Crystal structure of a putative immunoglobulin A1 protease (BACOVA_03286) from Bacteroides ovatus at 1.30 A resolution

Experimental procedure

Experimental method	MAD
Source type	SYNCHROTRON
Source details	SSRL BEAMLINE BL11-1
Synchrotron site	SSRL
Beamline	BL11-1
Temperature [K]	100
Detector technology	CCD
Collection date	2010-04-07
Detector	MARMOSAIC 325 mm CCD
Wavelength(s)	0.91162,0.97935,0.97899
Spacegroup name	P 43 21 2
Unit cell lengths	75.679, 75.679, 132.101
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	28.699 - 1.300
R-factor	0.137
Rwork	0.135
R-free	0.15700
Structure solution method	MAD
RMSD bond length	0.013
RMSD bond angle	1.520
Data reduction software	MOSFLM
Data scaling software	SCALA (3.3.15)
Phasing software	SHELX
Refinement software	REFMAC (5.5.0110)

Data quality characteristics

	Overall
Low resolution limit [Å]	28.699
High resolution limit [Å]	1.300
Number of reflections	94721
Completeness [%]	99.9
Redundancy	4

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	9	277	2.400000000M (NH4)2SO4, 0.1M Bicine pH 9.0, NANODROP, VAPOR DIFFUSION, SITTING DROP, temperature 277K