3N6Y

Crystal structure of an immunoglobulin-like protein (PA1606) from PSEUDOMONAS AERUGINOSA at 1.50 A resolution

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRL BEAMLINE BL9-2
Synchrotron site	SSRL
Beamline	BL9-2
Temperature [K]	100
Detector technology	CCD
Collection date	2010-05-13
Detector	MARMOSAIC 325 mm CCD
Spacegroup name	P 1 21 1
Unit cell lengths	41.073, 65.341, 53.065
Unit cell angles	90.00, 103.19, 90.00

Refinement procedure

Resolution	28.621 - 1.500
R-factor	0.182
Rwork	0.181
R-free	0.21200
Structure solution method	SAD
RMSD bond length	0.015
RMSD bond angle	1.534
Data reduction software	MOSFLM
Data scaling software	SCALA (3.3.15)
Phasing software	SHELX
Refinement software	REFMAC (5.6.0073)

Data quality characteristics

	Overall
Low resolution limit [Å]	28.621
High resolution limit [Å]	1.500
Number of reflections	43788
Completeness [%]	100.0
Redundancy	3.7

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	6.6	277	0.200000000M Na2SO4, 20.000000000% PEG-3350, No Buffer pH 6.6, NANODROP, VAPOR DIFFUSION, SITTING DROP, temperature 277K