3N6X
Crystal structure of a Putative glutathionylspermidine synthase (Mfla_0391) from METHYLOBACILLUS FLAGELLATUS KT at 2.35 A resolution
Experimental procedure
| Experimental method | MAD |
| Source type | SYNCHROTRON |
| Source details | SSRL BEAMLINE BL9-2 |
| Synchrotron site | SSRL |
| Beamline | BL9-2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2010-01-23 |
| Detector | MARMOSAIC 325 mm CCD |
| Wavelength(s) | 0.91837,0.97927,0.97911 |
| Spacegroup name | P 41 21 2 |
| Unit cell lengths | 97.344, 97.344, 155.487 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 29.940 - 2.350 |
| R-factor | 0.179 |
| Rwork | 0.178 |
| R-free | 0.20100 |
| Structure solution method | MAD |
| RMSD bond length | 0.010 |
| RMSD bond angle | 0.730 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | SOLVE |
| Refinement software | PHENIX |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 29.944 | 2.430 |
| High resolution limit [Å] | 2.350 | 2.350 |
| Rmerge | 0.104 | 0.833 |
| Number of reflections | 31751 | |
| <I/σ(I)> | 10.98 | 1.8 |
| Completeness [%] | 99.4 | 95.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 6 | 293 | 0.8000M ammonium sulfate, 0.1M MES pH 6.0, Additive: 0.003 M adenosine 5'-triphosphate magnesium salt, NANODROP', VAPOR DIFFUSION, SITTING DROP, temperature 293K |
