3N6U
Effector binding domain of TsaR in complex with its inducer p-toluenesulfonate
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU RUH2R |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2010-02-28 |
| Detector | MAR scanner 300 mm plate |
| Wavelength(s) | 1.54179 |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 51.500, 95.710, 82.940 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 25.780 - 1.870 |
| R-factor | 0.22195 |
| Rwork | 0.220 |
| R-free | 0.26655 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.024 |
| RMSD bond angle | 2.082 |
| Refinement software | REFMAC (5.5.0102) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 25.780 | 1.970 |
| High resolution limit [Å] | 1.870 | 1.870 |
| Rmerge | 0.027 | 0.166 |
| Number of reflections | 17327 | |
| <I/σ(I)> | 20.23 | 4.75 |
| Completeness [%] | 99.7 | 98.3 |
| Redundancy | 1.88 | 1.87 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 4.6 | 293 | 1.5M ammonium sulfate, 0.1M sodium acetate pH4.6, VAPOR DIFFUSION, SITTING DROP, temperature 293K |
