3N6L

The crystal structure of RNA-dependent RNA polymerase of EV71 virus

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PHOTON FACTORY BEAMLINE BL-17A
Synchrotron site	Photon Factory
Beamline	BL-17A
Temperature [K]	100
Detector technology	CCD
Collection date	2009-09-21
Detector	ADSC QUANTUM 270
Wavelength(s)	1.0000
Spacegroup name	P 32 2 1
Unit cell lengths	103.450, 103.450, 132.243
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	50.000 - 2.600
R-factor	0.22206
Rwork	0.219
R-free	0.26892
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.017
RMSD bond angle	1.757
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	REFMAC (5.2.0019)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	89.440	2.700
High resolution limit [Å]	2.600	2.600
Rmerge	0.065	0.408
Number of reflections	25646
Completeness [%]	95.0	95

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP			VAPOR DIFFUSION, HANGING DROP
1	VAPOR DIFFUSION, HANGING DROP			VAPOR DIFFUSION, HANGING DROP