3N5D
Crystal structure of the complex of type I ribosome inactivating protein with glucose at 1.9A resolution
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE BM14 |
Synchrotron site | ESRF |
Beamline | BM14 |
Temperature [K] | 77 |
Detector technology | CCD |
Collection date | 2010-05-12 |
Detector | MARRESEARCH |
Wavelength(s) | 0.97 |
Spacegroup name | H 3 |
Unit cell lengths | 130.666, 130.666, 40.498 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 65.330 - 1.900 |
R-factor | 0.19608 |
Rwork | 0.193 |
R-free | 0.25049 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1aha |
RMSD bond length | 0.023 |
RMSD bond angle | 1.993 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | AMoRE |
Refinement software | REFMAC (5.5.0072) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 65.330 | |
High resolution limit [Å] | 1.900 | 1.900 |
Number of reflections | 18830 | |
<I/σ(I)> | 29.6 | 1.9 |
Completeness [%] | 97.6 | 81.6 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6.7 | 298 | 14% PEG6000, 0.1M Sodium Phosphate, pH 6.7, VAPOR DIFFUSION, HANGING DROP, temperature 298K |