3N5B
The complex of PII and PipX from Anabaena
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL17U |
| Synchrotron site | SSRF |
| Beamline | BL17U |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2010-03-25 |
| Detector | RAYONIX MX-225 |
| Wavelength(s) | 0.9194 |
| Spacegroup name | P 3 2 1 |
| Unit cell lengths | 70.645, 70.645, 88.544 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 50.000 - 1.900 |
| R-factor | 0.20014 |
| Rwork | 0.198 |
| R-free | 0.24507 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1qy7 |
| RMSD bond length | 0.015 |
| RMSD bond angle | 1.530 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALA |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.5.0109) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 88.540 | 1.970 |
| High resolution limit [Å] | 1.900 | 1.900 |
| Rmerge | 0.070 | 0.360 |
| Number of reflections | 20553 | |
| <I/σ(I)> | 27.2 | 4.2 |
| Completeness [%] | 99.7 | |
| Redundancy | 8.8 | 5.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8.5 | 289 | 1.6M ammonium sulfate, 0.1M Tris-Cl pH8.5, 6.4% v/v Glycerol, VAPOR DIFFUSION, HANGING DROP, temperature 289K |
