3N5A
Synaptotagmin-7, C2B-domain, calcium bound
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-BM |
| Synchrotron site | APS |
| Beamline | 19-BM |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2009-04-10 |
| Detector | SBC-3 |
| Wavelength(s) | 1.0148 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 35.598, 44.892, 87.383 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 23.510 - 1.441 |
| R-factor | 0.161 |
| Rwork | 0.160 |
| R-free | 0.18100 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1tjm |
| RMSD bond length | 0.021 |
| RMSD bond angle | 1.340 |
| Data reduction software | HKL-3000 |
| Data scaling software | HKL-3000 |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.6_289) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 43.700 | 1.460 |
| High resolution limit [Å] | 1.440 | 1.440 |
| Rmerge | 0.039 | 0.397 |
| Number of reflections | 25581 | |
| <I/σ(I)> | 31.1 | 2.6 |
| Completeness [%] | 98.0 | 91 |
| Redundancy | 4.7 | 4.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 293 | 25% PEG 3350, 0.2 M Li2SO4, 0.1 M Hepes pH 6.5, 0.05 M NaCl, 0.02 M CaCl2, 1 mM TCEP, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
