3N53
Crystal structure of a response regulator receiver modulated diguanylate cyclase from Pelobacter carbinolicus
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X29A |
| Synchrotron site | NSLS |
| Beamline | X29A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2009-11-07 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.979 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 34.004, 44.932, 88.681 |
| Unit cell angles | 90.00, 94.37, 90.00 |
Refinement procedure
| Resolution | 44.210 - 2.200 |
| R-factor | 0.252 |
| Rwork | 0.252 |
| R-free | 0.28600 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1nxo |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.300 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | MOLREP |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 44.210 | 2.280 |
| High resolution limit [Å] | 2.200 | 2.200 |
| Rmerge | 0.075 | 0.243 |
| Number of reflections | 13639 | |
| <I/σ(I)> | 13.1 | 2.5 |
| Completeness [%] | 98.9 | 95.1 |
| Redundancy | 6.8 | 5.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6.5 | 298 | 0.2M Ammonium acetate, 0.1M Bis-Tris, 25% PEG 3350, pH 6.5, VAPOR DIFFUSION, SITTING DROP, temperature 298.0K |
