3N52
crystal Structure analysis of MIP2
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU |
| Temperature [K] | 93.1 |
| Detector technology | IMAGE PLATE |
| Detector | RIGAKU RAXIS IV++ |
| Wavelength(s) | 1.5418 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 41.755, 59.446, 99.354 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 35.000 - 1.900 |
| R-factor | 0.18785 |
| Rwork | 0.186 |
| R-free | 0.22553 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.030 |
| RMSD bond angle | 2.459 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.5.0109) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 50.000 | 50.000 | 1.680 |
| High resolution limit [Å] | 1.620 | 3.490 | 1.620 |
| Rmerge | 0.042 | 0.032 | 0.380 |
| Number of reflections | 30727 | ||
| <I/σ(I)> | 41.6 | ||
| Completeness [%] | 95.3 | 99.2 | 66.4 |
| Redundancy | 4.6 | 4.9 | 2.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8.5 | 291 | 0.1M Tris pH 8.5, 38% PEG 3350, VAPOR DIFFUSION, HANGING DROP, temperature 291K |
