3N4Y
Crystal structure of wild-type T. celer L30e in low ionic strength condition without precipitant
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU MICROMAX-007 |
| Temperature [K] | 110 |
| Detector technology | IMAGE PLATE |
| Collection date | 2009-06-30 |
| Detector | RIGAKU RAXIS IV |
| Wavelength(s) | 1.5418 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 24.179, 53.307, 33.733 |
| Unit cell angles | 90.00, 109.12, 90.00 |
Refinement procedure
| Resolution | 17.346 - 2.400 |
| R-factor | 0.187 |
| Rwork | 0.177 |
| R-free | 0.27790 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1h7m |
| RMSD bond length | 0.006 |
| RMSD bond angle | 0.893 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | MOLREP |
| Refinement software | PHENIX |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 31.870 | 2.530 |
| High resolution limit [Å] | 2.400 | 2.400 |
| Rmerge | 0.126 | 0.264 |
| Total number of observations | 8769 | |
| Number of reflections | 3199 | |
| <I/σ(I)> | 6.7 | 3.9 |
| Completeness [%] | 87.1 | 82.2 |
| Redundancy | 1.9 | 1.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | MICRODIALYSIS | 6.5 | 298 | 5mM sodium citrate, 5mM sodium phosphate, pH 6.5, MICRODIALYSIS, temperature 298K |
