3N4K
Putative RNA methyltransferase from Yersinia pestis in complex with S-ADENOSYL-L-HOMOCYSTEINE.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-ID |
| Synchrotron site | APS |
| Beamline | 19-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2010-03-14 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.9792 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 58.718, 72.275, 86.819 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 40.500 - 1.760 |
| R-factor | 0.163 |
| Rwork | 0.161 |
| R-free | 0.19300 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3n4j |
| RMSD bond length | 0.019 |
| RMSD bond angle | 1.710 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.5.0109) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 40.400 | 1.790 |
| High resolution limit [Å] | 1.760 | 1.760 |
| Rmerge | 0.064 | 0.764 |
| Number of reflections | 37358 | |
| <I/σ(I)> | 10.8 | 1.99 |
| Completeness [%] | 100.0 | 99.9 |
| Redundancy | 6.9 | 4.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6.5 | 289 | 0.1 M Bis-Tris, 20 % PEG MME 5000, 10 mM S-ADENOSYL-L-HOMOCYSTEINE, pH 6.5, VAPOR DIFFUSION, SITTING DROP, temperature 289K |
