3N4G
Crystal structure of native Cg10062
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU RU200 |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2005-01-29 |
| Detector | RIGAKU RAXIS IV |
| Wavelength(s) | 1.5418 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 104.097, 40.690, 89.938 |
| Unit cell angles | 90.00, 94.96, 90.00 |
Refinement procedure
| Resolution | 25.000 - 2.440 |
| R-factor | 0.211 |
| Rwork | 0.209 |
| R-free | 0.25900 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3n4d |
| RMSD bond length | 0.021 |
| RMSD bond angle | 1.799 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | CaspR |
| Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 50.000 | 50.000 | 2.520 |
| High resolution limit [Å] | 2.430 | 5.230 | 2.430 |
| Rmerge | 0.059 | 0.037 | 0.170 |
| Number of reflections | 14190 | ||
| <I/σ(I)> | 23.5 | ||
| Completeness [%] | 99.0 | 96.1 | 99.1 |
| Redundancy | 3 | 3 | 2.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 4 | 298 | 6 micro liter hanging drop consisting of equal volume of crystallization solution (3% PEG 6000, pH 4.0) and protein solution (18.1mg/L Cg10062 in 10mM Tris-SO4, pH 8.0), Hanging drop, temperature 298K |
