3N3F

Crystal Structure of the Human Collagen XV Trimerization Domain: A Potent Trimerizing Unit Common to Multiplexin Collagens

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU RU200
Temperature [K]	100
Detector technology	IMAGE PLATE
Collection date	2009-11-19
Detector	RIGAKU RAXIS
Wavelength(s)	1.54
Spacegroup name	P 63
Unit cell lengths	49.120, 49.120, 65.600
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	35.690 - 2.005
Rwork	0.216
R-free	0.25000
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3hsh
RMSD bond length	0.005
RMSD bond angle	1.097
Data reduction software	MOSFLM
Data scaling software	SCALA (3.3.15)
Phasing software	AMoRE
Refinement software	CNS

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	42.539	2.010
High resolution limit [Å]	2.005	2.005
Rmerge	0.034	0.110
Number of reflections	6095
<I/σ(I)>	35.4	15.6
Completeness [%]	100.0	100
Redundancy	6.6	6.5

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.5	298	0.1M Tris, 0.2 M MgCl2, 30% (w/v) PEG3350, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 298K